质粒名称: | CHOP promoter (-649/+136) pmCherry-1 |
---|---|
出品公司: | 爱迪基因 |
目录编号: | 36035 |
存储实验室: | Quan Lu |
载体骨架基本信息 |
|
载体名称: | pmCherry-1 |
载体出品公司: | Clontech |
载体抗性: | 卡那霉素 |
空载体大小: | 4140 bp |
完整质粒大小: | 4927 bp |
载体修饰: | XhoI and BamHI cleaved, removing intervening sequence in the MCS. |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
|
插入基因名称: | CHOP promoter (-647 to +136) |
物种来源: | H. sapiens (human) |
插入大小: | 787 bp |
基因又名: | CHOP |
GenBank编号 | NT_029419.12 NM_001195053 |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | XhoI(未破坏) |
3' 克隆位点: | BamHI (未破坏) |
5' 测序引物 | custom: GGCCTTTTGCTCACATGTTC |
文献引用方法 |
|
材料和方法部分: | CHOP promoter (-649/+136) pmCherry-1 was a gift from Quan Lu (爱迪基因 plasmid # 36035) |
参考文献部分: | Functional RNA Interference (RNAi) Screen Identifies System A Neutral Amino Acid Transporter 2 (SNAT2) as a Mediator of Arsenic-induced Endoplasmic Reticulum Stress. Oh RS, Pan WC, Yalcin A, Zhang H, Guilarte TR, Hotamisligil GS, Christiani DC, Lu Q. J Biol Chem. 2012 Feb 17;287(8):6025-34. Epub 2012 Jan 3. 10.1074/jbc.M111.311217 PubMed 22215663 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS CHOP promoter (-649/+136) pmCherry-1 4880 bp ds-DNA circular SYN 10-JAN-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS CHOP promoter (-649/+136) pmCherry-1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4880) AUTHORS Oh RS, Pan WC, Yalcin A, Zhang H, Guilarte TR, Hotamisligil GS, Christiani DC, Lu Q TITLE Functional RNA Interference (RNAi) Screen Identifies System A Neutral Amino Acid Transporter 2 (SNAT2) as a Mediator of Arsenic-induced Endoplasmic Reticulum Stress. JOURNAL J Biol Chem. 2012 Feb 17;287(8):6025-34. Epub 2012 Jan 3. PUBMED 22215663 COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTNAME|CHOP promoter (-649/+136) pmCherry-1| FEATURES Location/Qualifiers source 1..4880 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 831..840 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 837..1547 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(982..1000) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1250..1269 /label=mCherry-F /note="mCherry, forward primer" polyA_signal 1668..1789 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1705..1724) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1759..1778 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1796..2251) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1933..1954) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 2145..2164 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2278..2382 /gene="bla" /label=AmpR promoter promoter 2384..2741 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2407..2427) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2592..2727 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2654..2673 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2776..3570 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2830..2849) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3440..3459 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3758..3777) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3802..3849 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 4178..4766 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4667..4686 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 tagttattac tagcgctacc ggactcagat ctcgagatgt cgacaatccc agtggatgga 61 taccaacttt aaaaagaaaa gttcaaaagg cctatgtgcc cattagctgg gaggggccaa 121 gaaatatggg agtcccttat agtgggggta aaacggcggg taaagctagg tgggcggaac 181 agcagcttct gggggagaca agcggcaaag aggctcacga ccgactaggg gcgaccaagg 241 ctgatagccg ttggggccgt tgggcgccgg gagctggcgc cccgccctct ctcctctccc 301 ccaccctccg cacctcccac caccctcggt gtcccctgcg cgtgcgcgtg cagacaccgg 361 ttgccaaaca ttgcatcatc cccgcccccc tttcctcccc tccccccccg ctacactccc 421 ctccgcgcgc gcgcatgact cacccacctc ctccgtgaag cctcgtgacc caaagccact 481 tccgggtccg acactacgtc gaccccctag cgagagggag cgacgggggc ggtgccgcgg 541 ggctcctgag tggcggatgc gagggacggg gcgggaccaa tgccggcgtg ccactttctg 601 attggtaggt tttggggtcc cgcccctgag aggagggcaa ggccatggta aaagattaca 661 gccaggcgct cccgaggtca gagacttaag tctaaggcac tgagcgtatc atgttaaaga 721 tgagcgggtg gcagcgacag agccaaaatc agagctggaa cctgaggaga gagtgttcaa 781 gaaggaagtg tatcttcata catcaccaca cctgacggga tccaccggtc gccaccatgg 841 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 901 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 961 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 1021 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 1081 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 1141 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 1201 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 1261 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc 1321 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 1381 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 1441 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 1501 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtagcgg ccgcgactct 1561 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 1621 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 1681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 1741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa ggcgtaaatt 1801 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt 1861 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg 1921 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc 1981 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca 2041 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga 2101 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa 2161 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc 2221 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc 2281 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 2341 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc 2401 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa 2461 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc 2521 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc 2581 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct 2641 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga 2701 agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca agagacagga 2761 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 2821 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 2881 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 2941 gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 3001 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 3061 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 3121 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 3181 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 3241 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 3301 gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 3361 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3421 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3481 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3541 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3601 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3661 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3721 tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag gagacaatac 3781 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac ggtgttgggt 3841 cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat accccaccga 3901 gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac cccccaagtt 3961 cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc atagcctcag 4021 gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg 4081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact 4141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 4201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 4261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 4321 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 4381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 4441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 4501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 4561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 4621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 4681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 4741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 4801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 4861 accgtattac cgccatgcat //
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇:ph7SK-gRNA
- 下一篇:SIRT6 Flag